Includes bibliographical references and index.

Part I. Assembly of oligonucleotides in synthons. Building block synthesis using the polymerase chain assembly method / Julie A. Marchand and Jean Peccoud. Oligonucleotide assembly in yeast to produce synthetic DNA fragments / Daniel G. Gibson. Topdown real-time gene synthesis / Mo Chao Huang ... [et al.] De novo DNA synthesis using single-molecule PCR / Tuval Ben Yehezekel, Gregory Linshiz, and Ehud Shapiro -- Part II. Synthon assembly. SLIC : a method for sequence- and ligation-independent cloning / ‡r Mamie Z. Li and Stephen J. Elledge. ‡t Assembly of standardized DNA parts using bioBrick ends in E. coli / ‡r Olivia Ho-Shing ... [et al.] ‡t Assembling DNA fragments by USER fusion / ‡r Narayana Annaluru ... [et al.] ‡t Fusion PCR via novel overlap sequences / ‡r Kamonchai Cha-aim ... [et al.] ‡t Using recombineering to generate point mutations : the oligonucleotide-based "Hit and Fix" method / ‡r Suhwan Chang, Stacey Stauffer, and Shyam K. Sharan. ‡t Using recombineering to generate point mutations : galK-based positive-negative selection method / ‡r Kajal Biswas, Stacey Stauffer, and Shyam K. Sharan. ‡t Assembling large DNA segments in yeast / ‡r Héloïse Muller ... [et al.] ‡t Recursive construction of perfect DNA molecules and libraries from imperfect oligonucleotides / ‡r Gregory Linshiz, Tuval Ben Yehezkel, and Ehud Shapiro. ‡t Cloning whole bacterial genomes in yeast / ‡r Gwynedd A. Benders. ‡t Production of infectious poliovirus from synthetic viral genomes / ‡r Jeronimo Cello and Steffen Muller -- ‡g Part III. ‡t Software for gene synthesis. ‡t In silico design of functional DNA constructs / ‡r Alan Villalobos, Mark Welch, and Jeremy Minshull. ‡t Using DNAworks in designing oligonucleotides for PCR-based gene synthesis / ‡r David Hoover. ‡t De novo gene synthesis design using TmPrime software / ‡r Mo-Huang Li ... [et al.] ‡t Design-a-gene with GeneDesign / ‡r Sarah M. Richardson ... [et al.] -- ‡g Part IV. ‡t Education and security. ‡t Leading a successful iGEM team / ‡r Wayne Materi. ‡t The Build-a-Genome course / ‡r Eric M. Cooper ... [et al.] ‡t DNA synthesis security / ‡r Ali Nouri and Christopher F. Chyba.

AnnotationœbThis volume details methods for fabricating DNA, including techniques for the assembly of oligonucleotide, cloning of synthons into larger fragments,protocols, software and educational and biosecurity impacts of gene synthesis.